After injury or surgical intervention, platelets are natural reservoirs of growth factors and cytokines that play a critical role in healing through the formation, maturation, and repair of soft and hard tissues. Platelet concentrates have been used to improve such healing in multiple applications throughout medicine and dentistry since the 1940s. This course will review the underlying mechanisms of action of leukocyte-rich platelet rich fibrin (L-PRF) and the step-by-step protocols for L-PRF preparation from venipuncture through placement into patients.